Homogalacturonan
General | Antibody | Glycoprotein | Glycolipid | Enzyme | Reference8 antibodies registered for EP0500
| Name | 2F4 |
|---|---|
| Species | Mouse |
| Category | Monoclonal |
| Isotype | IgG1 |
| Immunogen | |
| Recognition region | Homogalacturonan |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [4][3] |
| Flow cytometry | |
| Histochemistry | |
| Availability | Plant Probes |
| Application | Immunodot assay[3] |
| Comment | Randomly de-esterified pectin was recognized by the antibody if its degree of methylesterification was <30% , whereas blockwise de-esterified pectin was recognized up to 40% of methylesterification.[4] |
| Name | JIM5 |
| Species | Rat |
| Category | Monoclonal |
| Isotype | IgG2a |
| Immunogen | |
| Recognition region | MeGalA(a1-4)GalA(a1-4)GalA(a1-4)GalA(a1-4)GalA(a1-4)MeGalA -R |
| Immunoprecipitation | |
| Immunoblot | [9][10] |
| ELISA/RIA | [2][7][9][6][8] |
| Flow cytometry | |
| Histochemistry | [11][9][10][12][6][13] |
| Availability | CarboSouse Plant Probes |
| Application | Immunodot assay[2][11][9][10] Microarray[14] |
| Comment | The JIM5 optimally binds to at least four contiguous unesterified residues between or adjacent to a metyl-ester group.[7] JIM5 binds to unesterified GalA residues with adjacent or flanking metyl-esterified residues.[7] |
| Name | JIM7 |
| Species | Rat |
| Category | Monoclonal |
| Isotype | IgA |
| Immunogen | |
| Recognition region | GalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)GalA(a1-4)-R GalA(a1-4)GalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)MeGalA(a1-4)GalA(a1-4)-R MeGalA(a1-4)GalA(a1-4)MeGalA(a1-4)GalA(a1-4)MeGalA(a1-4)GalA(a1-4)-R |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [2][7][6][8] |
| Flow cytometry | |
| Histochemistry | [5][6] |
| Availability | CarboSouse |
| Application | Immunodot assay[2][5] |
| Comment | JIM7 binds to metyl-esterified residues with adjacent or flanking unesterified GalA residues.[7] |
| Name | LM18 |
| Species | LM18 |
| Category | Monoclonal |
| Isotype | IgG2c |
| Immunogen | |
| Recognition region | Homogalacturonan |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [6] |
| Flow cytometry | |
| Histochemistry | [6] |
| Availability | Plant Probes |
| Application | |
| Comment | LM18 can bind to Homogalacturonan with few or no esters.[6] |
| Name | LM19 |
| Species | Rat |
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | |
| Recognition region | Homogalacturonan |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [6][8] |
| Flow cytometry | |
| Histochemistry | [6] |
| Availability | Plant Probes |
| Application | |
| Comment | LM19 can bind to Homogalacturonan with few or no esters.[6] |
| Name | LM20 |
| Species | Rat |
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | |
| Recognition region | partially methyl-esterified Homogalacturonan |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [6][8] |
| Flow cytometry | |
| Histochemistry | [6] |
| Availability | Plant Probes |
| Application | |
| Comment | LM20 require some Homogalacturonan methyl-esters for recognition.[6] |
| Name | LM7 |
| Species | Rat |
| Category | Monoclonal |
| Isotype | IgM |
| Immunogen | |
| Recognition region | partially Me-Homogalacturonan/ non-blockwise |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [11][7] |
| Flow cytometry | |
| Histochemistry | |
| Availability | Plant Probes |
| Application | Immunodot assay[11] |
| Comment | LM7 binds to unesterified GalA residues with adjacent or flanking metyl-esterified residues.[7] |
| Name | PAM1 |
| Species | - |
| Category | Single chain Fv |
| Isotype | |
| Immunogen | |
| Recognition region | blockwise de-esterifield Homogalacturonan |
| Immunoprecipitation | |
| Immunoblot | |
| ELISA/RIA | [1] |
| Flow cytometry | |
| Histochemistry | [1][11] |
| Availability | Plant Probes |
| Application | Immunodot assay [1][2][11] Microarray[14] |
| Comment | Optimal binding required in the region of 30 de-estrified GalA residues.[1] |